In parallel with the prevalence metabolic syndrome, nonalcoholic fatty liver disease (AFLD) has become the most common chronic liver disease in most countries. It features a constellation of simple steatosis, nonalcoholic steatohepatitis (NASH), fibrosis, cirrhosis, and even hepatocellular carcinoma. There are no approved drugs for effective management of NAFLD and NASH. Jianpi Huoxue formula (JPHX) mainly consists of Atractylodes macrocephal (Baizhu), Salvia miltiorrhiza (Danshen), Rasux Paeonia Alba (Baishao), Rhizoma Alismatis (Zexie), and Fructus Schisandrae Chinensis (Wuweizi), which may have beneficial effects on NAFLD. The aim of the study was to identify the effect of JPHX on NAFLD. A NAFLD model was induced by methionine-choline-deficient food (MCD) in Wistar rats, and orally administered with simultaneous JPHX, once a day for 8 weeks. Hepatocellular injury, lipid profile, inflammation, fibrosis and apoptosis were evaluated. The results showed that JPHX significantly decreased the abnormal serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels compared with the MCD model (P<0.05). Furthermore, IPHX protected MCD diet-fed rats from accumulation of hepatic VRSD triglycerides (TG)and total cholesterol (TC). Histological examination demonstrated NVEDQ that IPHX noticeably normalized the NAFLD activity score (NAS). Moreover, IPHX ameliorated liver inflammation by decreasing TNF-a levels and reduced collagen and matrix metalloproteinases in MCD diet-fed rats. In addition, JPHX prevented rats from MCD-induced cellular apoptosis, as suggested by TUNEL staining, and suppressed the activation of caspase 3 and 7 proteins. JPHX also inhibited the phosphorylation of INK. In conclusion, JPHX exhibited a hepatoprotective effect against NAFLD in an MCD experimental model

隨著肥胖，糖尿病，高血脂和代謝性疾病發病率的上升，非酒精性脂肪肝在很 多國家己成為最常見的慢性肝臟病。非酒精性脂肪肝病可分為脂肪堆積，非酒 精性脂肪肝炎，纖維化，肝硬化甚至是肝癌。目前沒有藥物可以有效治療該疾 病。健脾活血方主要由白式，丹參，白芍，澤瀉，還有五味子組成，可能對該 疾病有益。這篇研究的目的是探究健脾活血方在非酒精性脂肪肝模型中的作用。 在威士塔大鼠中通過蛋氨酸膽鹼缺乏的飼料誘導非酒精性脂肪肝模型八與此同 時每日給予中藥健脾活血方持續八週。評估肝臟損傷，脂質，炎症，纖維化和 凋亡。發現顯示和蛋氨酸膽鹼缺乏的模型組相比健脾活血方顯著降低了異常升 高的谷丙轉氨酶和穀草轉氨酶。此外，健脾活血方保護肝臟免於甘油三酯和總 膽固醇的堆積。肝臟病理擥結果顯示健脾活血方使肝臟組織擥病理評分正常化。 再者，健脾活血方通過降低腫瘤壞死因子的含量和減少膠原和基質金屬蛋白酶 的表達改善肝臟炎症。另外，正如 TUNEL 染色結果顯示 ，健脾活血改善細胞 凋亡和抑制半光天冬酶了和7的表達。健脾活血方也抑制了JNK 蛋白的磷酸化。結論，健脾活血方對蛋氨酸膽鹼缺乏誘導的非酒精脂肪肝有肝臟保護作用。 關鍵字:非酒精性脂肪肝，健脾活血方，蛋氨酸-膽鹼缺乏飲食，肝保護作用