The rhizoma and radix of "Curcuma longa L." are traditionally used as two Chinese medicines, Jianghuang and Yujin, respectively (There are other origins of "Yujin", herein, it is only mean the radix of Curcuma longa L.). Curcuminoids and essential oil are considered as main fractions of Curcuma longa. Recent research showed that Curcuma longa has effects on anti-oxidant, anti-tumor, anti-inflammation and hepatoprotection. As recorded in China pharmacopoeia 2005, c urcumine is used as the marker for quality control of Jianghuang, and total volatile oil should not be less than 7%. Yujin has no quantitative items for its quality control. Therefore, comparison of chemical components in volatile oil from Jianghuang and Yujin is very important for quality control of the two Chinese medicines. The thesis contained four chapters: Chapter 1 is a review of Curcuma longa, including the research of chemical components, pharmacology and its quality control. Chapter 2 is focused on the isolation and purification of components in Jianghuang oil. Four pure compounds ar-turmerone, ar-curcumene, B- turmerone and a- turmeron were obtained, which were confirmed by UV, MS and NMR. The purity of them are 96%, 98%, 98% and 95%, respectively. A quality control method for Jianghuang and Yujin was developed in Chapter 3.1. Optimization of extraction: Pressurized liquid extraction (PLE) was used for extraction. The optimized conditions: solvent, methanol; temperature, 140 °C; particle size, 0.15-0.20 mm; static extraction time, 5 min; pressure, 1000 p.s.i.; static cycle, 1 and 60% of the flush volume. GC-MS condition: The column temperature was at 80 °C for injection, then programmed at 20 °C-min' to 120 °C, then at 1 °C-min' to 130 °C and held for 5 min, then at 4 °C-min' to 160 °C, finally, at 20 °C min' to 280 °C. Split injection (2 ml) was conducted with a split ratio of 10:1 and high purity helium was used as carrier gas of 1.0 ml min" flow-rate. Results: Eight compounds were identified based on their MS data. Their contents in Jianghuang and Yujin from five different origins were determined. Chemical comparison between Jianghuang and Yujin was performed in Chapter 4. The results showed that Yujin has low contents of ar-curcumen and ar-turmerone. Cluster analysis showed that Jianghuang and Yujin were divided into two groups based on 4 compounds ar-curcumen, ar- turmerone, a-turmerone and B-turmerone. Therefore, ar-curcumen, ar-turmerone, a- turmerone and 3- turmerone could be used as markers for quality control of Jianghuang and Yujin.

整科植物薑黄 Curcuma longa L的根荃和塊根.被不儒中华的“斑查”的根 莖和塊根,被稱爲中藥的 “薑黄” (Rhizoma Curcumae Longae) 和“俳金” (Radix Curcumae)（“郁金“還有其他水源，木文中提到的“郁金”專指植物 葺黄的塊根）。薑黄素和揮發油被認焉是童黄的主要有效成分，現代硏究表明植物薑黄具有抗氧化、抗腫瘤、抗炎、抗微生物以及心血管保護作用。 2005 年版中國藥典中，薑黃是以薑黃素爲品質控制指標，對其揮發油只要 求總量不小於 7%：而郁金的品質控制，除水分、總灰分外，没有具體成分的含 量規定。本研究中，定性定量比較了薑黃和郁金，爲全面控制此兩種中藥的品質 提供了科學依據。全文共分四章：第一章是有關薑黃研究的文獻綜述，包括薑黃的化學成分和藥理學研究， 以及薑黃品質控制方法研究進展。 第二章爲薑黃揮發油主要化學成分的分離純化，共得到几個化合物，經UV，MS 和NMR 確認爲：芳薑黃酮 (ar-turmerone），芳薑黃烯 (ar-curcumene），B- 薑黄酮（B-turmerone)，a-薑黄酮 （a-turmeron）。其中B-薑黃酮和芳薑黃烯的 純度在 98%以上，芳薑黄酮和c-薑黃酮的純度分别爲 96%和11 95%。 第三章是應用 GC-MS 對薑黃和郁金的揮發油類成分定性定量分析品質控制 方法研究。包括： 1.薑黃與郁金藥材中揮發性成分的加壓溶劑提取條件（包括溶劑種類、藥 粉粒徑、提取溫度、提取壓力、提取時問、置換體積以及提取次數）的優化，結 果為：粒徑爲0.154-0.2mm 的藥材在壓強爲 1000Pa，溫度 140°C的條件下用甲醇 提取1次（5分鐘），置換體積 60%。 2. GC-MS條件（溫度、進樣量、分流比）的優化，最後探用梯度升溫(80 C-280°C），進樣24、分流比爲10:1。 3. 薑黃和郁金藥材中揮發油成分的定性定量分析。GC-MS 鑒定了8個成分並對5個不同產地的薑黃和郁金樂材的8種化合物進行定量。 第四章是薑黃和郁金藥材揮發油成分的比較分析，結果表明：8種化合物均 同時存在于薑黄和郁金藥材中，只是具體含量上有一定差别，尤其是芳薑黃酮和 芳躉黃烯差别較大。本硏究爲控制薑黃及郁金藥材揮發油部份的品質提供了科學 客觀的理論依據。