Lingzhi (reishi, ganoderma) is widely used in China, Japan, Korea and other East Asian countries. With a history of 2000 years, Lingzhi is considered to be a valuable health-maintaining food. Thanks to the new cultivation and liquid state fermentation technologies, the output of Lingzhi increased rapidly, and the effect of Lingzhi is widely recognized. More and more new researches of LZ on pharmacology, chemistry and cultivation were published. Most of the studies were focused on pharmacodynamics study of polysaccharide and triterpene. Studies on quality control of Lingzhi were limited. Ouality control is an important topic of Lingzhi, if it would be accepted worldwide. In this thesis, an analytical method was developed to assay nucleotides in LZ by HPLC-DAD-ESI-MS. It provided a useful analysis method, not only for scientific purposes but also for industrial applications. The researches included: HPLC-MS method was developed for the analysis of Lingzhi firstly. An analytical method was established for the determination of 9 nucleotides, which are present in the fruiting body of Lingzhi. The mobile phase was a linear gradient of 5 mm Ammonium acetate- methanoll By comparing two extraction methods, including ASE and sonication, sonication was chosen as the method to prepare the analysis samples. The repeatability, stability, calibration graph, LOD, precision, and recovery of the method were evaluated. The employed method was proved stable to analyze nucleotides in Lingzhi. The contents of nucleotides in different part of Lingzhi mushroom were determined. The results can offer useful information for utilizing Lingzhi. The nucleotide contents in other funguses were also analyzed to compare the contents of the 9 nucleotides. The result suggested that eleven nucleotides and nucleic bases presented in Ganoderma, including uridine, uracil, cytidine, thymine, thymidine, guanine, guanosine, adenine, adenosine, hypoxanthine and inosine. The content of uridine is the highest in Lingzhi, and followed by cytidine, guanosine and adenosine. The thymine and thymidine are limited. Further more, the content of nucleotide in Lingzhi is more than its corresponding base. At the same time, the sensitivity of the uracil and uridine was low in ESI mass detector. The studies also showed that the content of nucleotide in Zizhi is less than that in Chizhi, especially cytidine. Thymine and thymidine contained in the 5 determined funguses was very small. And it is presumed that there were little thymine and thymidine in fungus.

靈芝是一種在東南亞應用歷史悠久的傅統保健藥材，在中國應用歷史超過 2000年，隨著靈芝人工栽培和液態發酵技術的日漸進步，颠芝的來源得到很大拓展，應用日益廣泛，在保健和藥用方面的價值逐漸得到體現。 當前疑芝的研究涉及到了藥理、化學、深層發酵等方方面面，可以說獲得了 較大的成果，但多數集中在多糖和三萜的藥理研究上，盟芝的質量控制研究很少。這一直是制約消費者認同和接受靈芝的障礙，也是靈芝產品國際化的關鍵問題所在。本研究利用HPLC-DAD-ESI-MS技術，分析了靈芝核苷類成分的含量，建立了疑芝核苷類成分的定性、定量分析方法，爲靈芝的質量控制和質譜聯用技術在中藥成分分析中的應用提供了較大的參考價值，研究的內容主要包括： 1．首次在靈芝的定性定量分析中引入高效液相色譜-質譜聯用技術。用5mM 醋酸銨水溶液-甲醇體系建立了9種核苷類成分的定量分析方法，爲靈芝及其產品的質量控制提供了有益的資訊； 比較了超聲、加壓溶劑提取法等方式在提取靈芝核苷類成分中的優劣，建立了相對合適的樣品處理方法； 對靈芝的核苷類成分定量分析方法做了系統的方法學訴價(包括精密度、重複性、檢測限、定量限、工作曲線、回收率等)，結果表明該方法精密、準確、可靠，可用於分析靈芝中核苷類成分的含量； 比較了靈芝不同部位含量的差異，爲更有效的利用靈芝資源提供了依據。同時分析了其他真菌類植物中的核苷含量，比較了9種核苷的含量差異。 結果顯示：靈芝中含尿嘧啶、胞昔、次黄嘌呤、肌苷、胸腺嘧啶、胸昔、尿 苷、鳥嘌呤、鳥苷、腺嘌呤、腺苷這十一種核苷和城基。其中尿苷的含量最高，其次爲胞苷、鳥苷和腺苷，而胸腺嘧啶、胸苷和胞嘧啶的含量很少。核苷的含量都比它相對的城基含量要高很多。同時發現ESI-MS對尿嘧啶和尿苷的檢測靈敏度較低。 寶驗結果還證明：相對於赤芝，紫芝中的核苷含量相對較低，特别是胞苷的含量。檢測的5種真菌中胸腺嘧啶和胸苷的含量都較低或無法檢測到，結合文獻結果，可以初步推測胸腺嘧啶和胸苷在真菌類中含量較低。